pLV V5-lamin B2
(Plasmid
#175109)
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PurposeLentiviral expression of V5-tagged mouse Lmnb2
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-EF1a-IRES-Puro
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Backbone manufacturerTobias Meyer (Addgene plasmid # 85132)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLmnb2
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SpeciesM. musculus (mouse)
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Entrez GeneLmnb2
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV V5-lamin B2 was a gift from Larry Gerace (Addgene plasmid # 175109 ; http://n2t.net/addgene:175109 ; RRID:Addgene_175109)