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Addgene

pL-U6-sgRNA-SFFV-Puro-P2A-EGFP
(Plasmid #175037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pL-CRISPR.SFFV.GFP
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin ; eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sp sgRNA scaffold
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL-U6-sgRNA-SFFV-Puro-P2A-EGFP was a gift from Kristian Helin (Addgene plasmid # 175037 ; http://n2t.net/addgene:175037 ; RRID:Addgene_175037)

  • For your References section:

    MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells. Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K. Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4. 10.1038/s41467-021-23308-4 PubMed 34031396
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