pRepair-MPP8_mAID-P2A-BFP
(Plasmid
#175036)
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PurposeHomology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Vector typeHDR donor template
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Selectable markersBFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemAID-P2A-BFP
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRepair-MPP8_mAID-P2A-BFP was a gift from Kristian Helin (Addgene plasmid # 175036 ; http://n2t.net/addgene:175036 ; RRID:Addgene_175036) -
For your References section:
MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells. Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K. Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4. 10.1038/s41467-021-23308-4 PubMed 34031396
