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Addgene

pAS_4xMmaPylT(UCA)_EF1_MmaPylRS
(Plasmid #174899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Backbone manufacturer
    Addgene #140008 based on PB510B-1 (System Bioscience)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mma PylRS
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1392
  • Entrez Gene
    pylS (a.k.a. MSMAC_RS17105, MSMAC_3241)
  • Promoter EF1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xMmaPylT(UCA)_EF1_MmaPylRS was a gift from Simon Elsaesser (Addgene plasmid # 174899 ; http://n2t.net/addgene:174899 ; RRID:Addgene_174899)

  • For your References section:

    Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery. Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, Landreh M, Elsasser SJ. Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6. 10.1016/j.crmeth.2023.100626 PubMed 37935196
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