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Addgene

pAS_8xMmaPylT_EF1_FLAG-MmaPylRS
(Plasmid #174890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174890 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Backbone manufacturer
    Addgene #140008 based on PB510B-1 (System Bioscience)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mma PylRS
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1392
  • Mutation
    U25C PylT
  • Entrez Gene
    pylS (a.k.a. MSMAC_RS17105, MSMAC_3241)
  • Promoter EF1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_8xMmaPylT_EF1_FLAG-MmaPylRS was a gift from Simon Elsaesser (Addgene plasmid # 174890 ; http://n2t.net/addgene:174890 ; RRID:Addgene_174890)

  • For your References section:

    Engineered Human Induced Pluripotent Cells Enable Genetic Code Expansion in Brain Organoids. van Husen LS, Katsori AM, Meineke B, Tjernberg LO, Schedin-Weiss S, Elsasser SJ. Chembiochem. 2021 Aug 25. doi: 10.1002/cbic.202100399. 10.1002/cbic.202100399 PubMed 34431592
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