Skip to main content
Addgene

pSCARS1
(Plasmid #174868)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS414
  • Backbone size w/o insert (bp) 8835
  • Total vector size (bp) 9843
  • Vector type
    Bacterial Expression, Yeast Expression, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ARS1
  • Alt name
    Autonomous replicating sequence
  • Species
    Yarrowia lipolytica
  • Insert Size (bp)
    986
  • GenBank ID
    X68252.1

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tgagcgcgcgtaatacg
  • 3′ sequencing primer gatggcatccctaaatttgatgaaag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCARS1 was a gift from Zengyi Shao (Addgene plasmid # 174868 ; http://n2t.net/addgene:174868 ; RRID:Addgene_174868)

  • For your References section:

    Revisiting the unique structure of autonomously replicating sequences in Yarrowia lipolytica and its role in pathway engineering. Lopez C, Cao M, Yao Z, Shao Z. Appl Microbiol Biotechnol. 2021 Aug 6. pii: 10.1007/s00253-021-11399-4. doi: 10.1007/s00253-021-11399-4. 10.1007/s00253-021-11399-4 PubMed 34357429
Related items:
Commonly requested with: