pGL4.10/RSV_SF3B3-Xon_Luciferase
(Plasmid #174659)

• Purpose: Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.
• Depositing Lab: Beverly L. Davidson
• Publication: Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586-021-03770-2. doi: 10.1038/s41586-021-03770-2.

Backbone

• Vector backbone: pGL4.10[luc2]
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4242
• Total vector size (bp): 5859
• Modifications to backbone: Removed BamHI site in backbone plasmid
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xon cassette
• Species: H. sapiens (human)
• Insert Size (bp): 1193
• Mutation: Kozak ATG initiation sequence in middle exon
• Promoter: RSV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamhI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: TCCCGTCTTCGAGTGGGTA

Resource Information

• Supplemental Documents:
  • ADDGENE pGL4_10_RSV_SF3B3Xon_Luciferase.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry