Skip to main content
Addgene

VPS29-YFP
(Plasmid #174520)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    YFP-N1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VPS29
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    546
  • Entrez Gene
    VPS29 (a.k.a. DC15, DC7, PEP11)
  • Promoter CMV
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Ecor1 (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VPS29-YFP was a gift from Juan Bonifacino (Addgene plasmid # 174520 ; http://n2t.net/addgene:174520 ; RRID:Addgene_174520)

  • For your References section:

    Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7. Rojas R, van Vlijmen T, Mardones GA, Prabhu Y, Rojas AL, Mohammed S, Heck AJ, Raposo G, van der Sluijs P, Bonifacino JS. J Cell Biol. 2008 Nov 3;183(3):513-26. doi: 10.1083/jcb.200804048. 10.1083/jcb.200804048 PubMed 18981234
Related items:
Commonly requested with: