pET23 P53DBD
(Plasmid
#174503)
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Purposeexpress P53 DNA binding domain (94-312) in E.coli without tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET23a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3666
- Total vector size (bp) 4269
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameP53 DNA binding domain (corresponds to amino acid 94-312)
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Alt nameTP53
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SpeciesH. sapiens (human)
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Insert Size (bp)662
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Promoter T7
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Tag
/ Fusion Protein
- none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET23 P53DBD was a gift from Stewart Loh (Addgene plasmid # 174503 ; http://n2t.net/addgene:174503 ; RRID:Addgene_174503) -
For your References section:
Structure, function, and aggregation of the zinc-free form of the p53 DNA binding domain. Butler JS, Loh SN. Biochemistry. 2003 Mar 4;42(8):2396-403. doi: 10.1021/bi026635n. 10.1021/bi026635n PubMed 12600206
