pCS2-Jmjd3-F
(Plasmid #17440)

• Purpose: Expression of Jmjd3 in mammalian cells
• Depositing Lab: Kai Ge
• Publication: Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44.

Backbone

• Vector backbone: pCS2+ NLSMT
• Backbone size w/o insert (bp): 4400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: JMJD3
• Alt name: jumonji domain containing 3
• Species: M. musculus (mouse)
• Insert Size (bp): 5000
• GenBank ID: BC075632
• Entrez Gene: Kdm6b (a.k.a. 1700064E03Rik, Jmjd3)
• Tags / Fusion Proteins:
  • 6xMyc (N terminal on backbone)
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length mouse JMJD3 with a C-terminal FLAG was PCR-amplified from MGC clone BC075632 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.