pCS2-UTY-F
(Plasmid #17439)

• Purpose: Expression of UTY in mammalian cells
• Depositing Lab: Kai Ge
• Publication: Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44.

Backbone

• Vector backbone: pCS2+ NLSMT
• Backbone size w/o insert (bp): 4400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UTY
• Alt name: ubiquitously transcribed tetratricopeptide repeat gene, Y-linked
• Species: H. sapiens (human)
• Insert Size (bp): 4000
• GenBank ID: NM_007125
• Entrez Gene: UTY (a.k.a. UTY1)
• Tags / Fusion Proteins:
  • 6xMyc (N terminal on backbone)
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length human UTY with a C-terminal FLAG was PCR-amplified from human monocytic leukemia cell line THP-1 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.