pFX-mito-mKate2
(Plasmid #174178)

• Purpose: Express mKate2 with mitochondria-targeting sequence in mammalian cells
• Depositing Lab: Yusuke Ohba
• Publication: Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183-194. doi: 10.1247/csf.19028. Epub 2019 Nov 15.

Backbone

• Vector backbone: pFX
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4820
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mKate2 with mitochondria-targeting sequence
• Insert Size (bp): 800
• GenBank ID:
• Promoter: CMV
• Tag / Fusion Protein:
  • the mitochondria-targeting sequence of the cytochrome c oxidase subunit VIII (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry