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Addgene

SuExp His-Myc-hPLD3
(Plasmid #173851)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SuExp
  • Backbone manufacturer
    Nemazee lab (Deli)
  • Backbone size w/o insert (bp) 2000
  • Total vector size (bp) 3500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLD3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    intracellular and transmembrane domain truncated, with only extracellular domain
  • GenBank ID
    23646
  • Entrez Gene
    PLD3 (a.k.a. AD19, HU-K4, HUK4, SCA46)
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis, Myc, Factor X cleavable (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40 pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SuExp His-Myc-hPLD3 was a gift from David Nemazee (Addgene plasmid # 173851 ; http://n2t.net/addgene:173851 ; RRID:Addgene_173851)

  • For your References section:

    Cleavage of DNA and RNA by PLD3 and PLD4 limits autoinflammatory triggering by multiple sensors. Gavin AL, Huang D, Blane TR, Thinnes TC, Murakami Y, Fukui R, Miyake K, Nemazee D. Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w. 10.1038/s41467-021-26150-w PubMed 34620855
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