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Addgene

pPSU250
(Plasmid #173837)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173837 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pST50Tr
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
  • Species
    Synthetic
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BsrGI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPSU250 was a gift from Song Tan (Addgene plasmid # 173837 ; http://n2t.net/addgene:173837 ; RRID:Addgene_173837)
  • For your References section:

    The Penn State Protein Ladder system for inexpensive protein molecular weight markers. Santilli RT, Williamson JE 3rd, Shibata Y, Sowers RP, Fleischman AN, Tan S. Sci Rep. 2021 Aug 18;11(1):16703. doi: 10.1038/s41598-021-96051-x. 10.1038/s41598-021-96051-x PubMed 34408191