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PurposeThis retroviral vector expresses lacZ and has an IRES-NEO downstream
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBMN
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Backbone manufacturerNolan Lab
- Backbone size w/o insert (bp) 9798
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Vector typeRetroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLacZ-IRES-Neo
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBMN 5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This retroviral vector expresses lacZ and has an IRES-NEO downstream. Please note that the size of this plasmid has been found to differ from the sizes expected from the map and sequence information provided on this page.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMN-Z-IN was a gift from Garry Nolan (Addgene plasmid # 1735 ; http://n2t.net/addgene:1735 ; RRID:Addgene_1735)