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PurposeThis retroviral vector has an IRES-GFP downstream of the cloning sites
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBMN
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Backbone manufacturerNolan Lab
- Backbone size w/o insert (bp) 6332
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Growth instructionsGrow in Stbl3 at 30C
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameIRES-GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBMN 5' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The pBMN backbone with IRES allowing for expression of GFP. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
This plasmid is extremely slow growing and may need 2 days to grow.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMN-I-GFP was a gift from Garry Nolan (Addgene plasmid # 1736 ; http://n2t.net/addgene:1736 ; RRID:Addgene_1736)