LgBiT-hACE2
(Plasmid #173431)

• Purpose: protein expression plasmid of LgBiT-hACE2-IgG1 Fc
• Depositing Lab: David Nemazee
• Publication: Huang et al J Immunol. 2021 Jul 1;207(1):344-351. doi: 10.4049/jimmunol.2100155. Epub 2021 Jun 28.

Backbone

• Vector backbone: phCMV3
• Backbone size w/o insert (bp): 4200
• Total vector size (bp): 7300
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LgBiT-hACE2-IgG1 Fc
• Alt name: large bit of split luciferase, fused with human ACE2, human IgG1 Fc region
• Species: H. sapiens (human)
• Insert Size (bp): 3100
• GenBank ID: 59272
• Entrez Gene: ACE2 (a.k.a. ACEH)
• Promoter: CMV
• Tag / Fusion Protein:
  • IgG1 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40 pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry