Jag1-HA pIRES
(Plasmid #17336)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Backbone

• Vector backbone: pIRES
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 6100
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Jagged1
• Species: R. norvegicus (rat)
• Insert Size (bp): 3654
• Mutation: A silent C->G mutation at 3237
• GenBank ID: ENSRNOT00000010638 NM_019147
• Entrez Gene: Jag1
• Tag / Fusion Protein:
  • HA X3 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T3

Resource Information

• Addgene Notes:
  • Digest of 17336 with XbaI and NotI/EcoRI
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone of this plasmid appears to be approximately 600bp smaller than is reported. The depositing scientist is aware of this difference and reports that the plasmid functions as it should.