pCPRDEnv
(Plasmid #1732)

• Purpose: This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab
• Depositing Lab: Garry Nolan
• Publication: Nolan Lab Plasmids (unpublished)

Backbone

• Vector backbone: modified pUC119
• Backbone size w/o insert (bp): 12905
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Gag-Pol and Rev
• Species: Feline Immunodeficiency Virus

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: M13 reverse primer

Resource Information

• Supplemental Documents:
  • Nolan-felix
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Nolan plasmid Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.