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Addgene

pIRES(A7Mut)_CyOFP1_KCNQ1
(Plasmid #173162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES(A7Mut)_CyOFP1
  • Backbone size w/o insert (bp) 5502
  • Total vector size (bp) 7533
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNQ1
  • Alt name
    Kv7.1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2031
  • Entrez Gene
    KCNQ1 (a.k.a. ATFB1, ATFB3, JLNS1, KCNA8, KCNA9, KVLQT1, Kv1.9, Kv7.1, LQT, LQT1, RWS, SQT2, WRS)
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES2(A7Mut)-CyOFP1 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV_F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES(A7Mut)_CyOFP1_KCNQ1 was a gift from Al George (Addgene plasmid # 173162 ; http://n2t.net/addgene:173162 ; RRID:Addgene_173162)
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