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Addgene

pX330_Actb sgRNA / hSpCas9
(Plasmid #172832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172832 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330
  • Backbone size w/o insert (bp) 8484
  • Total vector size (bp) 8486
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330_Actb sgRNA / hSpCas9 was a gift from Haining Zhong (Addgene plasmid # 172832 ; http://n2t.net/addgene:172832 ; RRID:Addgene_172832)

  • For your References section:

    High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion. Zhong H, Ceballos CC, Massengill CI, Muniak MA, Ma L, Qin M, Petrie SK, Mao T. Elife. 2021 Jun 8;10. pii: 64911. doi: 10.7554/eLife.64911. 10.7554/eLife.64911 PubMed 34100715
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