pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1
(Plasmid
#172581)
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PurposeFor transient expression of C-terminal BirA-tagged MACROD1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1-MCS-BirA(R118G)-HA
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMACROD1
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Alt nameLRP16
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SpeciesH. sapiens (human)
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Insert Size (bp)978
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Entrez GeneMACROD1 (a.k.a. LRP16)
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Tag
/ Fusion Protein
- BirA(R118G)-HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV fw
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1 was a gift from Karla Feijs (Addgene plasmid # 172581 ; http://n2t.net/addgene:172581 ; RRID:Addgene_172581) -
For your References section:
Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome. Zaja R, Aydin G, Lippok BE, Feederle R, Luscher B, Feijs KLH. Sci Rep. 2020 May 19;10(1):8286. doi: 10.1038/s41598-020-64623-y. 10.1038/s41598-020-64623-y PubMed 32427867
