pET21b-LaG9(S151C)
(Plasmid #172490)

• Purpose: Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).
• Depositing Lab: Siegfried Musser
• Publication: Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. doi: 10.1038/s41556-021-00815-6. Epub 2022 Jan 10.

Backbone

• Vector backbone: pET21b
• Backbone manufacturer: Novagen (EMD Millipore)
• Backbone size w/o insert (bp): 5442
• Total vector size (bp): 5850
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LaG9(S151C)
• Species: Lama glama
• Insert Size (bp): 435
• Mutation: S151C
• GenBank ID: none
• Promoter: T7
• Tags / Fusion Proteins:
  • pelB leader sequence (N terminal on insert)
  • PPX cleavage site (C terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: T7 Terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Micheal Rout (Rockefeller University)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original report of the unmodified nanobody clone: Fridy, et al. (2014) A robust pipeline for rapid production of versatile nanobody repertoires. Nat. Meth. 11:1253-1260. Used and submitted with permission.