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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLNCX
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAkt1
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SpeciesM. musculus (mouse)
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MutationConstitutively active
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Entrez GeneAkt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
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Tag
/ Fusion Protein
- Myr (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymyristoylated Akt1 gene from P. Tsichlis
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLNCX myr Akt1 was a gift from Joan Brugge (Addgene plasmid # 17245 ; http://n2t.net/addgene:17245 ; RRID:Addgene_17245) -
For your References section:
Functional role and oncogene-regulated expression of the BH3-only factor Bmf in mammary epithelial anoikis and morphogenesis. Schmelzle T, Mailleux AA, Overholtzer M, Carroll JS, Solimini NL, Lightcap ES, Veiby OP, Brugge JS. Proc Natl Acad Sci U S A. 2007 Mar 6. 104(10):3787-92. 10.1073/pnas.0700115104 PubMed 17360431