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Addgene

pLenti-Ef1a-hMGT-PGK-puro
(Plasmid #172394)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172394 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti-GFP
  • Backbone manufacturer
    Eric Campeau & Paul Kaufman (Addgene plasmid # 17448)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mef2c, Gata4, Tbx5
  • Species
    H. sapiens (human)
  • Entrez Gene
    MEF2C (a.k.a. C5DELq14.3, DEL5q14.3, NEDHSIL)
  • Entrez Gene
    GATA4 (a.k.a. ASD2, TACHD, TOF, VSD1)
  • Entrez Gene
    TBX5 (a.k.a. HOS)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Ef1a-hMGT-PGK-puro was a gift from Li Qian (Addgene plasmid # 172394 ; http://n2t.net/addgene:172394 ; RRID:Addgene_172394)
  • For your References section:

    An Optimized Protocol for Human Direct Cardiac Reprogramming. Garbutt TA, Zhou Y, Keepers B, Liu J, Qian L. STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2019.100010. Epub 2020 Jun 3. 10.1016/j.xpro.2019.100010 PubMed 32728671