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Addgene

pMKO shRNA Bim
(Plasmid #17235)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMKO.1
  • Backbone manufacturer
    Available at Addgene (#8452)
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bim shRNA
  • Alt name
    Bim
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • Entrez Gene
    BCL2L11 (a.k.a. BAM, BIM, BOD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer pLXSN 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

see article supplementary materials for shRNA seequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKO shRNA Bim was a gift from Joan Brugge (Addgene plasmid # 17235 ; http://n2t.net/addgene:17235 ; RRID:Addgene_17235)

  • For your References section:

    Functional role and oncogene-regulated expression of the BH3-only factor Bmf in mammary epithelial anoikis and morphogenesis. Schmelzle T, Mailleux AA, Overholtzer M, Carroll JS, Solimini NL, Lightcap ES, Veiby OP, Brugge JS. Proc Natl Acad Sci U S A. 2007 Mar 6. 104(10):3787-92. 10.1073/pnas.0700115104 PubMed 17360431
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