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pAGHa-SERBP1(189-400)
(Plasmid #172315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 5200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Plasminogen Activator Inhibitor 1 RNA-binding protein, residues 189-400
  • Alt name
    SERBP1, Serpine1 mRNA Binding Protein 1, PAI-RBP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    699
  • Mutation
    deleted 1-188, 401-408
  • Entrez Gene
    SERBP1 (a.k.a. CGI-55, CHD3IP, HABP4L, PAI-RBP1, PAIRBP1)
  • Tag / Fusion Protein
    • His8

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KasI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGHa-SERBP1(189-400) was a gift from David Libich (Addgene plasmid # 172315 ; http://n2t.net/addgene:172315 ; RRID:Addgene_172315)

  • For your References section:

    The (1)H, (15)N and (13)C resonance assignments of the C-terminal domain of Serpine mRNA binding protein 1 (SERBP1). Baudin A, Xu X, Libich DS. Biomol NMR Assign. 2021 Oct;15(2):461-466. doi: 10.1007/s12104-021-10046-3. Epub 2021 Aug 26. 10.1007/s12104-021-10046-3 PubMed 34436734
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