pAG8Ha-SERBP1(149-400)
(Plasmid #176516)

• Purpose: Expresses the human C-terminal SERBP1 149-400 construct.
• Depositing Lab: David Libich
• Publication: Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707. doi: 10.3389/fmolb.2021.744707. eCollection 2021.

Backbone

• Vector backbone: pAG8Ha
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5760
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SERBP1 149-400
• Alt name: SERPINE1 mRNA binding protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 760
• Mutation: deleted amino acids 1-148 and 401-408
• GenBank ID: NM_001018067.2
• Entrez Gene: SERBP1 (a.k.a. CGI-55, CHD3IP, HABP4L, PAI-RBP1, PAIRBP1)
• Promoter: T7
• Tag / Fusion Protein:
  • His8 tag, linker, TEV site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KasI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry