pEx-deletion-tet
(Plasmid
#172214)
-
Purpose(Empty Backbone) For allelic exchange in tetracyclin-sensitive Prevotella copri strains
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepExchange-tdk
-
Backbone manufacturerKoropatkin, N.M. (PMID: 18611383)
- Backbone size (bp) 4172
-
Vector typeAllelic exchange in Prevotella copri
-
Selectable markersGentamicin ; Tetracycline in Prevotella copri
Growth in Bacteria
-
Bacterial Resistance(s)Amp + DAP
-
Growth Temperature37°C
-
Growth Strain(s)β2155
-
Growth instructionsSupplemented Media with 0.3 mM 2,6-Diaminopimelic acid (DAP)
-
Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEx-deletion-tet was a gift from Till Strowig (Addgene plasmid # 172214 ; http://n2t.net/addgene:172214 ; RRID:Addgene_172214) -
For your References section:
A versatile genetic toolbox for Prevotella copri enables studying polysaccharide utilization systems. Li J, Galvez EJC, Amend L, Almasi E, Iljazovic A, Lesker TR, Bielecka AA, Schorr EM, Strowig T. EMBO J. 2021 Dec 1;40(23):e108287. doi: 10.15252/embj.2021108287. Epub 2021 Oct 21. 10.15252/embj.2021108287 PubMed 34676563
