pEx-deletion-erm
(Plasmid #172213)

• Purpose: (Empty Backbone) For allelic exchange in erythromycin-sensitive Prevotella copri strains
• Depositing Lab: Till Strowig
• Publication: Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi: 10.15252/embj.2021108287. Epub 2021 Oct 21.

Backbone

• Vector backbone: pExchange-tdk
• Backbone manufacturer: Koropatkin, N.M. (PMID: 18611383)
• Backbone size (bp): 4172
• Vector type: Allelic exchange in Prevotella copri
• Selectable markers: Erythromycin in Prevotella copri

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): S17lambdapir
• Growth instructions: Supplementing Media with 0.3 mM 2,6-Diaminopimelic acid (DAP) is required if grown in B2155 E.coli strain
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.