pET28-AviTag-DogCatcher-MBP
(Plasmid #171928)

• Purpose: Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
• Depositing Lab: Mark Howarth
• Publication: Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 10.1016/j.chembiol.2021.07.005.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6932
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AviTag-DogCatcher-MBP
• Species: Synthetic
• Insert Size (bp): 1569
• Mutation: DogCatcher added to N-terminus of MBP via a GSGESG linker
• Promoter: T7
• Tags / Fusion Proteins:
  • His6 (N terminal on backbone)
  • Thrombin cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry