pOPINB-hRNF216(457-784)
(Plasmid #171922)

• Purpose: Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.
• Depositing Lab: Bernhard Lechtenberg
• Publication: Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. doi: 10.1016/j.molcel.2021.12.005. Epub 2022 Jan 7.

Backbone

• Vector backbone: pOPINB
• Backbone size w/o insert (bp): 5642
• Total vector size (bp): 6297
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E3 ubiquitin-protein ligase RNF216
• Alt name: RNF216
• Alt name: TRIAD3A
• Species: H. sapiens (human)
• Insert Size (bp): 984
• Mutation: codon optimised for expression in E. coli
• GenBank ID: NM_207116
• Entrez Gene: RNF216 (a.k.a. CAHH, TRIAD3, U7I1, UBCE7IP1, ZIN)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6x-His tag (N terminal on backbone)
  • 3C protease cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • BCL1121_pOPINB_T7F_T7R.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry