M28 Nac>GFP
(Plasmid
#17178)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17178 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonen/a
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenacre promoter
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Alt namenacre
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)836
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GenBank IDAF211890
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Entrez Genemitfa (a.k.a. nacre, z3A.1)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note for Use:
Description: zebrafish nacre promoter driving GFP. A 836-bp fragment including the first 10 amino acids of nacre was fused to GFP.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M28 Nac>GFP was a gift from Randall Moon (Addgene plasmid # 17178 ; http://n2t.net/addgene:17178 ; RRID:Addgene_17178) -
For your References section:
Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway. Dorsky RI, Raible DW, Moon RT. Genes Dev. 2000 Jan 15;14(2):158-62. PubMed 10652270
Map uploaded by the depositor.
