pUHD-SB-mAID/Zeo
(Plasmid
#171681)
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Purpose(Empty Backbone) TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (zeocin)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSBbi
- Backbone size (bp) 4835
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Vector typeMammalian Expression
- Promoter TRE
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Selectable markersZeocin
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Tag
/ Fusion Protein
- mAID (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUHD-SB-mAID/Zeo was a gift from Randy Poon (Addgene plasmid # 171681 ; http://n2t.net/addgene:171681 ; RRID:Addgene_171681) -
For your References section:
One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines. Yeung TK, Lau HW, Ma HT, Poon RYC. Mol Biol Cell. 2021 May 12:mbcE21020051. doi: 10.1091/mbc.E21-02-0051. 10.1091/mbc.E21-02-0051 PubMed 33979199
