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pJCC_084 Cas12i1 D647A
(Plasmid #171671)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171671 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2CT
  • Backbone manufacturer
    UC Berkeley MacroLab
  • Backbone size w/o insert (bp) 5972
  • Total vector size (bp) 9251
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas12i1 D647A
  • Insert Size (bp)
    3279
  • Mutation
    changed aspartate 647 to alanine
  • Tags / Fusion Proteins
    • 10X His (N terminal on backbone)
    • maltose-binding protein (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Arbor Biotechnologies, Addgene plasmid 120882 (pET28a-mH6-Cas12i1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJCC_084 Cas12i1 D647A was a gift from Jennifer Doudna (Addgene plasmid # 171671 ; http://n2t.net/addgene:171671 ; RRID:Addgene_171671)

  • For your References section:

    CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks. Cofsky JC, Karandur D, Huang CJ, Witte IP, Kuriyan J, Doudna JA. Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143. 10.7554/eLife.55143 PubMed 32519675
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