pJCC_076 AsCas12a R1226A
(Plasmid #171669)

• Purpose: For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag
• Depositing Lab: Jennifer Doudna
• Publication: Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143.

Backbone

• Vector backbone: p2CT
• Backbone manufacturer: UC Berkeley MacroLab
• Backbone size w/o insert (bp): 5969
• Total vector size (bp): 9890
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AsCas12a R1226A
• Alt name: AsCpf1 R1226A
• Species: Acidaminococcus sp.
• Insert Size (bp): 3921
• Mutation: changed arginine 1226 to alanine
• Tags / Fusion Proteins:
  • 10X His (N terminal on backbone)
  • maltose-binding protein (N terminal on backbone)
  • TEV protease cleavage site (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pJCC_076 AsCas12a R1226A
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid 113430 (pMBP-AsCas12a)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry