pVP65KR-gb-SacB(-)
(Plasmid #171646)

• Purpose: Equipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.
• Depositing Lab: Young Kee Chae
• Publication: Chae et al Protein Pept Lett. 2021;28(12):1398-1407. doi: 10.2174/0929866528666211105113750.

Backbone

• Vector backbone: pVP65K
• Backbone manufacturer: Center for Eukaryotic Structural Genomics (CESG)
• Selectable markers: 5% sucrose

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MBP-mCherry-8xHis-SacB(-)
• Mutation: internal HindIII site in the SacB gene was removed.
• Tag / Fusion Protein:
  • 8xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SgfI (unknown if destroyed)
• 3′ cloning site: PmeI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pVP65KR-gb-SacB(-).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Korean patent: Auto-induction Plasmid, 10-2286426.
For more information, please see Chae YK (2021) FASEB J, https://doi.org/10.1096/fasebj.2021.35.S1.01779