M16 pCS-twhh-bGFP
(Plasmid #17153)

• Depositing Lab: Randall Moon
• Publication: Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Backbone

• Vector backbone: pCS

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: twhh promoter
• Alt name: tiggy-winkle hedgehog
• Species: D. rerio (zebrafish)
• Insert Size (bp): 5200
• Entrez Gene: shhb (a.k.a. twh, twhh)
• Tag / Fusion Protein:
  • bGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: twhh promoter (5.2 Kb), bGFP (1 Kb)

Plasmid History:

The reporter gene, "bright" green fluorescent protein (bGFP) with a serine 65 to threonine mutation (Heim et al., 1995), was cloned into vector pCS-twhh-beta -gal-vec BamHI/XhoI sites by blunt end ligation. The resulting plasmid was named pCS-twhh-bGFP.

Notes for Use:
Gives notochord-specific expression of GFP.
Citation: Du et al., 1997, J. Cell Biology.