LV-AR-C563A-C596A
(Plasmid #171220)

• Purpose: Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.
• Depositing Lab: Steven Balk
• Publication: Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi: 10.1038/s41467-021-27077-y.

Backbone

• Vector backbone: Lenti-viral-Hygro
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AR-C563A-C596A
• Alt name: AR Zinc Finger mutant
• Alt name: AR ZF mutant
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: AR-C563A-C596A
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: EF-1a
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mutations occur on one essential cysteine in each of the two zinc fingers, leading to complete loss of AR DNA binding capacity.

Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.