LV-AR-AAFF
(Plasmid #171218)

• Purpose: Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.
• Depositing Lab: Steven Balk
• Publication: Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi: 10.1038/s41467-021-27077-y.

Backbone

• Vector backbone: Lenti-viral-Hygro
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AR-AAFF
• Alt name: AR-K580A-V581A
• Alt name: AR P-box mutant
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: AR-K580A-V581A
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: EF-1a
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mutations occur on the androgen receptor KVFF motif in the P-box of the zinc finger 1 that make contact with DNA. AR mutants of the KVFF motif would lose activity to activate AR-mediated transcription.

Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.