Berger Lab Human Paralog Knockout Library (pgPEN)
(Pooled Library #171172)

• Purpose:

  The human pgPEN lentiviral CRISPR library induces knockouts of paralogous genes. Plasmids in the library express a pair of two guide RNAs (pgRNAs). Each construct knocks out an individual gene or pair of paralogs.

• Vector Backbone:

  lentiGuide-Puro (Plasmid #52963) - does not express Cas9

• Depositing Labs: Alice Berger
• Publication: Parrish et al Cell Rep. 2021 Aug 31;36(9):109597. doi: 10.1016/j.celrep.2021.109597.

Library Details

• Species: Human
• Genes targeted: 1,030 duplicate paralog gene pairs
• gRNAs:
  
  • 8 pgRNAs for single gene knockout
  • 16 pgRNAs for combined knockouts
• Controls: 500 non-targeting paired gRNAs
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • The depositor recommends following the GeCKo v2.0 Library Amplification Protocol (PDF, 276 KB)
  • PCR protocol for paired guide RNA library NGS preparation (PDF, 240 KB)
• Depositor Data:
  • List of targeted genes and paired guide sequences (XLSX, 1.8 MB)
  • NGS Read Counts (TXT, 2.0 MB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.