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Addgene

pMSCVhyg-3xT7
(Plasmid #171028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCVhyg
  • Vector type
    Mammalian Expression, Retroviral
  • Promoter 5' LTR
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • 3x-T7 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTAG1-hygroTK-N-term 3xT7 double StrepII tag

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Brown DA, Di Cerbo V, Feldmann A, Ahn J, Ito S, Blackledge NP,
Nakayama M, McClellan M, Dimitrova E, Turberfield AH,
et al. 2017. The SET1 complex selects actively transcribed target
genes viamultivalent interaction with CpG island chromatin.
Cell Rep 20: 2313–2327. doi:10.1016/j.celrep.2017.08.030

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCVhyg-3xT7 was a gift from Kai Ge (Addgene plasmid # 171028 ; http://n2t.net/addgene:171028 ; RRID:Addgene_171028)

  • For your References section:

    MED1 is a lipogenesis coactivator required for postnatal adipose expansion. Jang Y, Park YK, Lee JE, Wan D, Tran N, Gavrilova O, Ge K. Genes Dev. 2021 Apr 22. pii: gad.347583.120. doi: 10.1101/gad.347583.120. 10.1101/gad.347583.120 PubMed 33888555
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