POLArIS_NbVim-cp10GFP
(Plasmid
#171020)
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PurposeExpresses NbVim-cp10GFP#2 in mammalian cells
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171020 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemodified pcDNA3.1
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 3300
- Total vector size (bp) 4400
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Modifications to backbonecontains Ribosome Binding Site (RBS) and N-terminal Histag for expression in E.coli.
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
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SpeciesSynthetic
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Insert Size (bp)1140
- Promoter CMV promoter
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Tag
/ Fusion Protein
- cp10GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV promoter
- 3′ sequencing primer BGH polyA (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMaier et al. Sci Rep . 2015 Aug 21;5:13402. doi: 10.1038/srep13402 (Figure 1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
POLArIS_NbVim-cp10GFP was a gift from Sumio Terada (Addgene plasmid # 171020 ; http://n2t.net/addgene:171020 ; RRID:Addgene_171020) -
For your References section:
Development of nanobody-based POLArIS orientation probes enabled multi-color/multi-target orientation imaging in living cells. Nakai N, Sato K, Tani T, Kawagishi M, Ka H, Saito K, Terada S. Biochem Biophys Res Commun. 2021 Jun 2;565:50-56. doi: 10.1016/j.bbrc.2021.05.088. 10.1016/j.bbrc.2021.05.088 PubMed 34090210
