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ZE36 Nlk1(C387Y)-pCS2+
(Plasmid #17077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nlk1
  • Alt name
    nlk
  • Alt name
    nemo-like kinase
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1425
  • Mutation
    C387Y. Inactive mutant of NLK1.
  • GenBank ID
    AY562552
  • Entrez Gene
    nlk1 (a.k.a. nlk, nlkb, zgc:111875)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Clone is an inactive mutant of NLK1, analogous to C425Y mutant of mouse NLK which does not bind TCFs.

Species of Sequence Origin: zebrafish.

Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.

Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE36 Nlk1(C387Y)-pCS2+ was a gift from Randall Moon (Addgene plasmid # 17077 ; http://n2t.net/addgene:17077 ; RRID:Addgene_17077)

  • For your References section:

    nemo-like kinase is an essential co-activator of Wnt signaling during early zebrafish development. Thorpe CJ, Moon RT. Development. 2004 Jun;131(12):2899-909. doi: 10.1242/dev.01171. Epub 2004 May 19. 10.1242/dev.01171 PubMed 15151990
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