pUC19-eGFP-P2A-dTAG-Ing3
(Plasmid #170754)

• Purpose: Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus
• Depositing Lab: Kristian Helin
• Publication: Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi: 10.1016/j.xpro.2021.100575. eCollection 2021 Jun 18.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 5196
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP-P2A-2xHA-dTAG
• Alt name: eGFP-P2A-2xHA-FKBP12(F36V)
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 2400
• Mutation: Phenylalanine 36 to valine in FKBP12
• Promoter: none
• Tag / Fusion Protein:
  • eGFP-P2A-2xHA-dTAG

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13/pUC Reverse
• 3′ sequencing primer: M13/pUC Forward

Resource Information

• Supplemental Documents:
  • pUC19-eGFP-P2A-dTAG-Ing3 targeting vector.gbk
• A portion of this plasmid was derived from a plasmid made by: the P2A-2xHA-dTAG cassette was amplified by PCR from pCRIS-PITChv2-BSD-dTAG BRD4 vector (addgene #91792)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry