pStr-KDEL_TPV-CXCL9-mCherry-SBP
(Plasmid #170677)

• Purpose: Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.
• Depositing Lab: Ke Xu
• Publication: Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8. doi: 10.1016/j.devcel.2021.12.018. Epub 2022 Jan 19.

Backbone

• Vector backbone: Str-KDEL_SBP-EGFP-Ecadherin
• Backbone manufacturer: Franck Perez Lab (Addgene #65286)
• Backbone size w/o insert (bp): 4407
• Total vector size (bp): 6277
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CXCL9
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1246
• GenBank ID: NM_002416.2
• Entrez Gene: CXCL9 (a.k.a. CMK, Humig, MIG, SCYB9, crg-10)
• Promoter: IRES
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • SBP (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: IRES-F
• 3′ sequencing primer: SV40pA-R

Gene/Insert 2

• Gene/Insert name: streptavidin-KDEL
• Species: Synthetic
• Insert Size (bp): 624
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pStr-KDEL_TPV-CXCL9-mCherry-SBP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry