ZE1 Wnt-1 myc
(Plasmid
#17042)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17042 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSP64T
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Backbone manufacturerDoug Melton Lab (Addgene #15030)
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namewnt-1
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Alt namewnt1
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1200
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Entrez Genewnt1 (a.k.a. WNT-1, int-1, sb:eu647, zgc:194464, zgc:194478)
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Tag
/ Fusion Protein
- myc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert: wnt-1 myc.
Insert size: 1.2 Kb.
Transcription: linearize with Pst1, transcribe with Sp6.
Plasmid History:
Constructed by Greg Kelly on date of 1/27/93, page 20 Book 2.
Notes for Use:
There is also a construct where wnt-1 myc is clone in antisense orientation.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZE1 Wnt-1 myc was a gift from Randall Moon (Addgene plasmid # 17042 ; http://n2t.net/addgene:17042 ; RRID:Addgene_17042)