pBluescript-IC3-FLAG-Tev-ZZ
(Plasmid #170311)

• Purpose: Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.
• Depositing Lab: Andrew Carter
• Publication: Mali et al Science. 2021 Feb 26;371(6532):910-916. doi: 10.1126/science.abe0526.

Backbone

• Vector backbone: pBluescript
• Total vector size (bp): 7401
• Selectable markers: Paromomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Outer Dynein Arm Intermediate Chain 3
• Alt name: IC2
• Species: Tetrahymena thermophila
• Insert Size (bp): 7401
• GenBank ID: XM_001015764
• Tag / Fusion Protein:
  • FLAG, ZZ (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: actggccgtcgttttac
• 3′ sequencing primer: gtcctttgtcgatactg

Resource Information

• Supplemental Documents:
  • p1904_IC3_Flag_Tev_ZZ_neo3_3UTR.gb
• A portion of this plasmid was derived from a plasmid made by: The original plasmids were received from Dr Kazufumi Mochizuki's laboratory

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference for source of plasmid backbone:
Kataoka, K., Schoeberl, U.E., and Mochizuki, K. (2010). Modules for C-terminal epitope tagging of Tetrahymena genes. J. Microbiol. Methods 82, 342–346.