Skip to main content
Addgene

pGV3340
(Plasmid #170278)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170278 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5089
  • Total vector size (bp) 6396
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HIV-2 RT p51 (mutant)
  • Alt name
    HIV-2 reverse transcriptase, small subunit (mutant)
  • Species
    HIV-2
  • Promoter T7-lac
  • Tag / Fusion Protein
    • Hexahistidine (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGV3340 was a gift from Gregory Van Duyne (Addgene plasmid # 170278 ; http://n2t.net/addgene:170278 ; RRID:Addgene_170278)

  • For your References section:

    Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons. Sherrill-Mix S, Hwang Y, Roche AM, Glascock A, Weiss SR, Li Y, Haddad L, Deraska P, Monahan C, Kromer A, Graham-Wooten J, Taylor LJ, Abella BS, Ganguly A, Collman RG, Van Duyne GD, Bushman FD. Genome Biol. 2021 Jun 3;22(1):169. doi: 10.1186/s13059-021-02387-y. 10.1186/s13059-021-02387-y PubMed 34082799
Related items:
Commonly requested with: