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Addgene

pGV3319
(Plasmid #170277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3543
  • Total vector size (bp) 5313
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bst-LF D720A
  • Alt name
    Bst DNA polymerase, large fragment (D720A)
  • Species
    Bacillus stearothermophilus
  • Insert Size (bp)
    1770
  • Mutation
    D720A
  • Promoter T7-lac
  • Tag / Fusion Protein
    • Hexahistidine (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGV3319 was a gift from Gregory Van Duyne (Addgene plasmid # 170277 ; http://n2t.net/addgene:170277 ; RRID:Addgene_170277)

  • For your References section:

    Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons. Sherrill-Mix S, Hwang Y, Roche AM, Glascock A, Weiss SR, Li Y, Haddad L, Deraska P, Monahan C, Kromer A, Graham-Wooten J, Taylor LJ, Abella BS, Ganguly A, Collman RG, Van Duyne GD, Bushman FD. Genome Biol. 2021 Jun 3;22(1):169. doi: 10.1186/s13059-021-02387-y. 10.1186/s13059-021-02387-y PubMed 34082799
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